Figure 3.

S. flavescens extract increases the axon density at lesion sites in SCI mice. Sagittal sections of spinal cords were obtained from S. flavescens extract—or vehicle-treated SCI mice at 31 days after the injury. The sections were immunostained for 5-HT, GFAP, and CSPG. (A) Representative immunofluorescence images of 5-HT. The yellow solid lines indicate outlines of the spinal cords. The yellow dotted line surrounds the lesion sites identified using the immunofluorescence images of GFAP. (B–D) The 5-HT-positive area at the lesion site (B), size of the lesion site (C), and expression of CSPG at the lesion site (D) were quantified. The numbers in parentheses indicate the numbers of mice. ^*p < 0.05 vs. vehicle group, unpaired two-tailed t-test. Scale bar = 500 μm.