Figure 7.

Matrine, oxymatrine, and S. flavescens extract have no effects on axonal extension on the PDL coating. Cortical neurons were cultured for 1 day and then treated with matrine (1 and 10 μM), oxymatrine (1 and 10 μM), S. flavescens extract (1 and 10 μg/ml), or vehicle solution. Four days after the treatment, the cells were fixed and immunostained for pNF-H and MAP2. The density of pNF-H-positive axons per neuron were quantified for each treatment. The numbers in parentheses indicate the measured number of captured images.