Fig. 5.

Effect of Trx1 on the migration of MDA-MB-231 cells. (A) ROS levels in stable transfected MDA-MB-231 cells analyzed using H₂DCF-DA and represented as a fold change over pIRES-EGFP transfected cells. A one-way ANOVA followed by Sidak’s post test was employed and showed that cells over-expressing the 1SS redox inactive Trx1 protein exhibited significantly higher levels of ROS compared to the control cells, *p<0.05. Results are presented as mean±SEM of three independent experiments performed in triplicate. (B) Representative images of the transfected cells used in the monolayer scratch assays over a period of 48 h. The images were taken at 4× using a Tucsen TCA 3MP camera and the scale bar is 50 µm. (C) Migration of stable transfected cells in response to over-expression of wild-type Trx1 and the 1SS redox inactive Trx1 protein. A repeated-measures two-way ANOVA followed by Sidak’s post test was employed and showed a statistical difference at 48 hour time point between 1SS over-expressing cells and control cells, *p<0.0001. Results are presented as mean±SEM of at least three independent experiments performed in quadruplicate.