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. 2015 Dec 19;8:79–90. doi: 10.1016/j.redox.2015.12.006

Fig. 1.

Fig. 1

Effects of Nrf2 knockdown on melanogenesis in HEMn and B16F10 cells in response to UVA irradiation. (A) HEMn and B16F10 cells were transfected with 5 nM Nrf2-siRNA (siNrf2) or non-silencing siRNA control (siCtrl) for 48 h. mRNA levels of Nrf2 and its target antioxidants (GCLC, GCLM, GST and NQO1) of HEMn and B16F10 cells transfected with siNrf2 were evaluated by real-time RT-PCR. *P<0.05; **P<0.01; ***P<0.001 versus siCtrl-transfected cells. (B) Melanin content and (C) tyrosinase activity were measured in HEMn and B16F10 cells transfected with siNrf2 or siCtrl at 1 h following UVA (8 J/cm2) irradiation. (D) Tyrosinase protein expression was measured in HEMn and B16F10 cells transfected with siNrf2 or siCtrl at 24 h post-irradiation. Data was expressed as mean±SD. The statistical significance of differences was evaluated by one-way ANOVA followed by Dunnett’s test. #P<0.05; ##P<0.01 versus siCtrl-transfected cells without UVA irradiation. **P<0.01; ***P<0.001 versus siNrf2-transfected cells irradiated with UVA.