Fig. 3.
The protective effects of CA, FA, QU, RU and AV on UVA-induced oxidative damage and GSH depletion. (A) Oxidant formation was examined in B16F10 cells pretreated with test compounds at 1 h after UVA irradiation (8 J/cm2) and H2O2 (250 µM) treatment. Determination of DCFDA was performed by flow cytometry and data was expressed as a percentage of control (100%, non-irradiated and untreated cells). (B) 8-OHdG and (C) GSH levels were determined at 1 h after UVA irradiation. Data was expressed as mean±SD from at least three independent experiments. The statistical significance of differences between the control and UVA or H2O2-treated cells was evaluated by Student’s t test and between UVA or H2O2-treated and compounds-treated cells by one-way ANOVA followed by Dunnett’s test. ###P<0.001 versus unirradiated control cells. *P<0.05; **P<0.01; ***P<0.001 versus untreated cells subjected to UVA or H2O2.