Figure 2. Transcription of the 4E-BP and Tor genes under conditions extending longevity. (A-D) Effects of DR on levels of 4E-BP and Tor mRNA.

Average expression of mRNA for the 4E-BP (A, B) and Tor (C, D) genes in three biological replicates isolated from the heads (A, C) and thoraces (B, D) of wild type CS female flies kept on 0.5N and 1.5N food at 10 or 40 days of age by Q-PCR. *p<0.05, 25 heads or thoraces per replicate. (E- H) Effects of rpd3 mutations on the levels of 4E-BP and Tor mRNA (E) Average expression of mRNA for the 4E-BP gene isolated from the heads and thoraces of rpd3^def/+ and +/+ control male flies at 20 and 40 days of age by Q-PCR. (F) Average expression of mRNA for the 4E-BP gene isolated from thoraces of rpd3^P-UTR/+ and control rpd3^P-1.8/+ male flies at 20 or 40 days of age by Q-PCR. (G) Average expression of mRNA for the Tor gene isolated from the heads and thoraces of rpd3^def/+ and +/+ control male flies at 20 and 40 days of age by Q-PCR. (H) Average expression of mRNA for the Tor gene isolated from the thoraces of rpd3^P-UTR/+ and control rpd3^P-1.8/+ male flies at 20 and 40 days of age by Q-PCR. In each case three biological replicates were used. Graphs are plotted as the means +/− standard errors. *p<0.05 **p<0.01, 25 heads + thoraces or thoraces per replicate.