Table 6. Effect of DR on longevity of 4E-BP hypomorphic (Top) and control (Bottom) flies.
| Gender | Genotype | Food (N) | Number | Mean Lifespan (% Change to controls) | X2 | p | Maximal Lifespan (% Change) |
|---|---|---|---|---|---|---|---|
| M | Thork07736/+ | 1.0 | 209 | 42.8 | 66.1 | ||
| M | Thork07736/+ | 0.5 | 231 | 49.2 () | 16.914 | <0.001* | 66 (0) |
| M | Thork07736/+ | 0.7 | 221 | 47.6 () | 12.807 | 0.0003* | 69.8 (6) |
| M | Thork07736/+ | 1.5 | 201 | 41.8 () | 0.7227 | 0.3953 | 58.3 (−12) |
| M | Thork07736/+ | corn | 220 | 42.8 () | 80.044 | <0.001* | 79.0 (20) |
| F | Thork07736/+ | 1.0 | 226 | 38.3 () | 56.0 | ||
| F | Thork07736/+ | 0.5 | 231 | 54.6 () | 208.70 | <0.001* | 72 (29) |
| F | Thork07736/+ | 0.7 | 234 | 54.3 () | 191.31 | <0.001* | 74.5 (33) |
| F | Thork07736/+ | 1.5 | 220 | 37.5 () | 0.2207 | 0.6385 | 52.9 (6) |
| F | Thork07736/+ | corn | 222 | 59.4 () | 282.08 | <0.001* | 78.0 (40) |
| M | +/+ | 1.0 | 273 | 52.7 | 73.8 | ||
| M | +/+ | 0.5 | 286 | 51.7 | 1.745 | 0.1865 | 72.6 (2) |
| M | +/+ | 0.7 | 277 | 51.1 | 0.5569 | 0.4555 | 73.5 (0) |
| M | +/+ | 1.5 | 277 | 43.3 | 58.062 | <0.001* | 64.93 (−12) |
| M | +/+ | corn | 280 | 54.0 | 2.1704 | 0.1407 | 71.8 (−3) |
| F | +/+ | 1.0 | 273 | 57.6 | 74.2 | ||
| F | +/+ | 0.5 | 247 | 59.9 | 10.077 | 0.0015* | 78.9 (6) |
| F | +/+ | 0.7 | 282 | 52.5 | 28.028 | <0.001* | 71 9 (−4) |
| F | +/+ | 1.5 | 284 | 42.7 | 226.87 | <0.001* | 59.6 (−20) |
| F | +/+ | corn | 273 | 58.6 | 2.81 | 0.0937 | 75.9 (2) |
The mean and maximal lifespan of male (M) and female (F) 4E-BP (Thork07736/+) (Top) mutant and control (Bottom) +/+ flies on different food levels. All values are compared to values of flies the same sex and genotype kept on 1.0N food to determine the percent increase in mean and maximal lifespan. Food (N): standard food is 1.0N. Number: number of flies used in the experiment. Mean and maximal lifespan are in days. Data are censored for 0-10 days. Long-rank analyses were performed using the JMP 11 program.