Figure 1. NSun2 regulates p27 expression.

(A) HeLa cells were transfected with a vector expressing NSun2 (pNSun2) or with a siRNA targeting NSun2. Forty-eight hours later, cell lysates were prepared and subjected to Western blot analysis to assess the levels of proteins NSun2, p27, and GAPDH. (B) RNA prepared from cells described in Fig. 1A was used for RT-qPCR analysis to assess the levels of p27 mRNA. Data represent the means ± SD from 3 independent experiments.