Figure 6. NSun2 delays replicative senescence by regulating p27, CDK1, and CDC25C.

(A) 2BS cells were stably infected with a lentivirus bearing a pHBLV-NSun2 vector (pNSun2) or with a control lentivirus (Vector), or stably co-infected with a lentivirus bearing a pHBLV-luc-5′UTR vector (Luc-5′UTR) or kept untreated (Control). Cell lysates were prepared and subjected to Western blot analysis to assess the levels of proteins NSun2, luciferase, p27, CDK1, CDC25C, and GAPDH. (B) The cells described in Fig. 6A were analyzed for cell cycle distribution (left) and the percentage of cells in each cell cycle compartment represented (right). (C) Cells described in Fig. 6A were used for analysis of SA-β-gal activity (left). The means ± SD from 3 independent experiments are presented and the significance was assessed by using Student's t test (right).