Figure 3. Von Kossa staining for the analysis of mineralized bone nodule formation. Photomicrographs show that treatment with sodium hyaluronate (HY; B), single-walled carbon nanotubes (SWCNT; C), and HY-functionalized SWCNT (HY-SWCNT; D) increased the mineralization area compared with the control (A). Morphometric analysis (E) showed that treatment with HY and HY-SWCNT significantly increased the formation of mineralized bone nodules. *P<0.05 compared to control (one-way ANOVA followed by Newman-Keuls post-hoc test). Scale bar = 100 µm.