Fig. 2. Identification of heat stable allergens. Proteins (10 µg) before (N) and after (H) heat treatment were separated on a 10% polyacrylamide gel containing sodium dodecyl sulfate under reducing conditions. Proteins were stained with Coomassie Blue (A) or probed with pooled serum from silkworm allergic patients (B). Arrows indicate the heat stable IgE binding proteins.
