Fig. 3. Protein profile (A) and IgE reactive components (B) of silkworm pupa extract on two-dimensional gels. Proteins were visualized by Coomassie Blue staining (A) and IgE-reactive components were probed with pooled serum from silkworm-allergic subjects (B). The protein in the circle was excised and subjected to LC-coupled ESI-MS/MS analysis.
