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. 2016 Jan 13;7:11. doi: 10.1186/s13287-015-0268-x

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© Butenschön et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunocytochemistry and flow cytometric analysis of NPCs before and after MACS and FACS. Before MACS, 50.8 % of all cells were positive for PSA-NCAM (a) (pre sort) and 5.07 % positive for SSEA-1 (b) (pre sort). After MACS and FACS, immunofluorescence showed that cells are positive for PSA-NCAM (a) (post sort) and negative for SSEA-1 (b) (post sort). DAPI 4′,6-diamidino-2-phenylindol, NPC neural progenitor cells, PSA-NCAM polysialylated neural cell adhesion molecule, SSEA-1 stage-specific embryonic antigen-1