A) DFS-IIF pattern (FITC) in HEp-2-ANA slides produced by human anti-DFS sera (a representative serum is shown), rabbit antibody to DFS70/LEDGFp75, and rabbit antibody to MeCP2. Corresponding DAPI images are shown in black and white for better visualization of chromatin. B) Immunoblots showing the reactivity of human anti-DFS sera (two representative sera are shown), rabbit antibody to DFS70/LEDGFp75, rabbit antibody to MeCP2, and mouse monoclonal antibody to MeCP2, against whole protein lysates from PC3 and DU145 cells. Beta-actin was used as loading control.