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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Clin Immunol. 2015 Jul 31;161(2):241–250. doi: 10.1016/j.clim.2015.07.014

Figure 6. Immunoadsorption of anti-DFS sera with autoepitope-containing peptide.

Figure 6

A) Immunoblots showing the abolition of anti-DFS/LEDGFp75 reactivity against PC3 lysates in human anti-DFS sera preadsorbed with a peptide corresponding to the C-terminal autoepitope region of DFS70/LEDGFp75 (representative sera shown). Preadsorption of anti-MeCP2 antibodies with the autoepitope-containing peptide did not abrogate their immunoreactivity. Similarly, preadsorption of the rabbit anti-DFS70/LEDGFp75 antibody, which targets a region outside the autoepitope, did not abrogate its immunoreactivity. Beta-actin was used as loading control. B) Preadsorption of representative human anti-DFS serum with the autoepitope-containing peptide abolished its immunoreactivity (green) in HEp-2-ANA slides. However, preadsorption of rabbit anti-MeCP2 antibody did not abolish its immunoreactivity (red) in HEp-2-ANA slides. Corresponding DAPI images are shown in black and white for better visualization of chromatin.