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(A–D) EMSA analysis of Cus2p interactions with various U2 stem II core model RNAs. Each RNA was incubated with a variable amount of Cus2p (0 to 2 µM). A single prominent shift (Shift-1) was observed with the WT and stem IIa-stabilized RNAs (A,B), while the stem IIc and G53A RNAs (C,D) also produced a higher mobility shift (Shift-2).
