FIGURE 6.

Dominant-negative effects of hsPrp38^NTD+ on splicing. (A) In vitro splicing of MINX pre-mRNA monitored on a denaturing gel. Educts, intermediates, and products of the reactions are identified on the left. Lanes 1–5—time course of splicing (time points indicated above the gel) in the absence of hsPrp38^NTD+. Lanes 6–13—effects of adding increasing amounts of hsPrp38^NTD+ (lanes 6–9) or hsPrp38^NTD+,D145A (lanes 10–13; indicated above the gel) to the reaction. (B) Native gel monitoring the kinetics of spliceosome assembly (time points above the gel) in the absence (lanes 1–6) or presence of increasing amounts of hsPrp38^NTD+ (lanes 7–10) or hsPrp38^NTD+,D145A (lanes 11–14; indicated above the gel). Emerging complexes are identified on the left; H—hnRNP complexes; A, B, C—spliceosomal A, B, and C complexes.