Figure 7. 1,25D3-mediated VDR recruitment to the Cyp11a1 promoter region is altered in CD8⁺ T cells after adoptive transfer into sensitized and challenged CD8-deficient mice.

CD8⁺ T cells were differentiated in vitro in IL-2 in the presence or absence of 1 μM 1,25D3. CD8⁺ T cells from the lungs were recovered after adoptive transfer into sensitized and challenged CD8-deficient mice (n=3 mice per group). qPCR was performed using five Cyp11a1 promoter-specific primers covering seven VDR-binding sites. Data were analysed via the percent input methodology: (2^{CT of total input−CT of specific IP}) × 100.