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. Author manuscript; available in PMC: 2016 Jan 14.
Published in final edited form as: Pharm Anal Acta. 2015 Oct 9;6(10):424. doi: 10.4172/2153-2435.1000424

Figure 2.

Figure 2

Effects of OM on hyperoxia-induced cytotoxicity in HPMEC: Human pulmonary microvascular endothelial cells (HPMEC) treated with DMSO, OM 5 or OM 100 were exposed to air or hyperoxia for up to 48 h, following which: (A) Cell viability was assessed by MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) reduction activities; and (B) Cell proliferation was determined based on the measurement of cellular DNA content via fluorescent dye binding using the CyQUANT NF cell proliferation assay. Data are representative of at least three independent experiments. Values are presented as means ± SEM (n=3). Two-way ANOVA showed an effect of hyperoxia but not of OM treatment for the dependent variables, cell viability and proliferation, in this figure. Significant differences between air- and hyperoxia-exposed cells are indicated by *, p<0.05.