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Multiple DIA method precursor range and mass-isolation window sizes evaluated.
| DIA method | Precursor range (m/z) | Number of sequential DIA windows | Isolation width (m/z) | Duty cycle (sec) |
|---|---|---|---|---|
| 1 | 410–690 | 56 | 6 | 3.1 |
| 2 | 550–830 | 56 | 6 | 3.1 |
| 3 | 690–970 | 56 | 6 | 3.1 |
| 4 | 970–1250 | 56 | 6 | 3.1 |
| 5 | 550–750 | 40 | 6 | 4.3 |
| 6 | 410–550 | 70 | 3 | 3.9 |
| 7 | 550–690 | 70 | 3 | 3.9 |
| 8 | 690–830 | 70 | 3 | 3.9 |
| 9 | 830–970 | 70 | 3 | 3.9 |
| 10 | 970–1110 | 70 | 3 | 3.9 |
| 11 | 1110–1250 | 70 | 3 | 3.9 |
| 12 | 400–1200 | 32 | 26 | 3.4 |
