Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Visualizing diazo-labeled proteins with Fl-DIBO (1) as fluorogenic reagent. A. Diazo-labeling of BSA (20 mg/mL in PBS) using NHS-activated ester 12 (25 mM in DMSO) and subsequent reaction with Fl-DIBO (1) (250 µM) overnight at 37 °C. B. In-gel visualization of labeled BSA with either Fl-DIBO or DIBO-FITC by fluorescence imaging (top row; λ□□□ = 365 nm; λdetec = 480 nm) and by Coomassie Blue stain to reveal total protein content (bottom row).
