Fig. 2.

The PC1 CTT contains CTS activity but the coiled-coil motif is insufficient to function as a CTS. (A) Schematic representation of CD16.7 and different PC1 C-terminal fragment fusions generated in this study. (B) Expression of indicated constructs in A in the primary cilia of IMCD-3 cells. Cells were stained by antibodies against CD16 (green) and acetylated α-tubulin (Ac-α-tub, red). Scale bar: 5 µm. (C) Quantification of percentage of CD16-positive cilia in cells. Green columns represent VxP motif-containing constructs and red columns represent constructs without the VxP motif. At least 50 ciliated, transfected cells were counted for the presence of CD16 signal on cilia under each condition. Error bars represent the s.e.m. between microscope fields from at least three independent experiments.