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Functional modulation of mAChR-induced KCNQ2–mCit surface transport by CHIR99021 and TBB. (A,B) Time course (A) and summary histogram (B) showing that pretreatment with 5 µM CHIR99021 (CHIR) facilitated mAChR-induced KCNQ2–mCit surface transport and its blockade by 10 µM TBB. **P<0.01. (C) Application of 5 µM CHIR99021 alone did not affect the TIRF signal of KCNQ2–mCit. Error bars show s.e.m. n values are given in brackets on the graphs.
