Inhibition of myosin IIA induces multiple lumens in tubes. (A) Representative images of cysts and tubules stably expressing control (Scramble), myosin IIA or myosin IIB shRNA, and stained for myosin IIA (green), F-actin (red) and E-cadherin (white). Arrows indicate multiple lumens. Scale bars: 10 μm. (B) Immunoblot of myosin IIA or myosin IIB depletion. (C) Quantification of tubules with multiple lumens in control-, myosin-IIA- or myosin-IIB-depleted cells. Results are mean±s.d. (n=3). *P<0.01, **P<0.05 (Student's t-test).