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. 2015 Dec 1;128(23):4366–4379. doi: 10.1242/jcs.173518

Fig. 3.

Fig. 3.

sE-cad is necessary for induction of lumen filling in MDCK cysts. (A) Representative confocal images showing lumen filling and disrupted epithelial architecture induced by 10 μg/ml purified sE-cad over 24 h. (B) Quantification of lumen filling from three independent experiments. Results are mean±s.e.m. ***P<0.0001 (Student's t-test). (C) Immunoblot showing sE-cad levels in the conditioned medium before and after immunodepletion of sE-cad. Quantification data represent mean±s.e.m. from three independent experiments. Note that the immunodepleted sE-cad is bound to Protein G beads. (D) Representative confocal images of control cysts, conditioned medium (CM)-treated cysts and cysts treated with sE-cad-depleted conditioned medium. Cysts were stained for actin (red) and TOPRO-3 (nuclear marker, blue). (E) Quantification of lumen-filled cysts in presence of conditioned medium and sE-cad-depleted conditioned medium. Results are mean±s.e.m. for B,E and the blot shown in C. ***P<0.001 (Student's t-test).