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. 2015 Dec 1;128(23):4366–4379. doi: 10.1242/jcs.173518

Fig. 5.

Fig. 5.

Lumen filling is a consequence of reduced apoptosis and increased proliferation. (A) Immunofluorescence images showing increased Ki67 and Bcl2 expression in MDCK cysts treated with conditioned medium (CM) and sE-cad for 48 h. Images were obtained from staining cysts with anti-Bcl2 antibody (green), phalloidin–Alexa-Fluor-546 (actin, red) and Ki67 (blue). (B) Representative immunoblot showing Ki67, Bcl2 and cyclin D1 expression in MDCK cysts treated with sE-cad and conditioned medium. 1 µM CL-387,785 (CL) EGFR inhibitor was used where indicated. Quantification data represent mean±s.d. from two independent experiments.