Fig. 3.

The oligomerization and ATPase activity of PSMC5 are necessary for Shoc2–PSMC5 complex localization on late endosomes and/or MVBs. (A) Cos1 cells co-transfected with GST–PSMC5 and the Shoc2–tRFP truncated mutants depicted in Fig. S3A, were followed by live-cell fluorescence microscopy. (B) Cos-SR cells expressing the GST–PSMC5 mutants depicted in Fig. S3C were fixed, immunostained for GST and followed by immunofluorescence microscopy. (C) Cos-SR cells expressing the CFP–PSMC5 mutants depicted in Fig. S3C were followed by live-cell fluorescence microscopy. Insets show high magnification images of the regions of the cell indicated by white rectangles. Scale bars: 10 µm.