Skip to main content
. 2015 Dec 1;142(23):4038–4048. doi: 10.1242/dev.125674

Fig. 9.

Fig. 9.

Gdf5 knockdown inhibits endplate reinnervation in Dach2−/−/Hdac9−/− mice. (A) Quantification of the percentage of fully innervated endplates at 14 days post nerve crush (dpc) in Dach2−/−/Hdac9−/− mice whose denervated soleus muscles were electroporated with a CMV:GFP expression vector and a siRNA targeting either E. coli lacZ mRNA or mouse Gdf5 mRNA. Error bars are s.d.; n=3. ***P<0.001. (B) Representative images of CMV:GFP and siRNA electroporated Dach2−/−/Hdac9−/− muscle fibers. GFP (green) identifies electroporated fibers, αBtx (red) identifies muscle endplates and βIII tubulin (cyan) identifies motor nerves. Arrowheads point to partially innervated and denervated endplates, whereas arrows point to fully innervated endplates. Scale bar: 50 µm.