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. 2015 Dec 1;142(23):4026–4037. doi: 10.1242/dev.126649

Fig. 1.

Fig. 1.

miRNAs are dynamically regulated in response to heart resection injury. Total RNA was extracted from uninjured and 6 h post-amputation (hpa) ventricles. cDNAs were synthesized from small RNAs and hybridized onto microarray chips in triplicate, to detect changes in miRNA expression. (A) Heat-map of the microarray data shows dynamic upregulation and downregulation of miRNAs in response to cardiac injury (green indicates lower expression; red indicates increased expression). (B) Real-time qRT-PCR validation of a subset of the most highly differentially expressed miRNAs from uninjured and 6 hpa wild-type hearts. miRNA levels were normalized to U6 and represented as fold-change relative to expression levels in uninjured hearts. (C) Time course of miR-101a expression levels during heart regeneration. qPCR studies reveal that relative to uninjured hearts (red dashed line) miR-101a expression (blue line) is at its lowest levels at 3 days post-amputation (dpa), increases to 1.5-fold at 14 dpa and is restored to pre-injury levels by 30 dpa. *P<0.05, **P<0.001 (Student's t-test); error bars represent s.e.m.