Fig. 3.

Sustained suppression of miR-101a leads to increased scar tissue. (A-F) Wild-type and Tg(hs:miR-101a-sp) hearts were resectioned, heat treated daily and collected at 7, 14 and 30 dpa for histology. Hearts were sectioned at 10 µm and stained with Acid Fucshin Orange G (AFOG) to detect muscle (brown), collagen (blue) and fibrin (red). Arrowheads in F mark newly regenerated muscle; dashed lines indicate approximate resection injury plane. The penetrance of each phenotype is indicated (number showing the illustrated phenotype/total number of samples). (G) Scarring indices were established by quantifying the percentage of collagen and fibrin within the total injury area at the indicated time points using CellProfiler. Prolonged depletion of miR-101a led to greater scar tissue at 14 and 30 dpa compared with control. (H) Injury size was comparable between the groups as shown by quantification of total injury area. n=5-14; *P<0.001 (Student's t-test); error bars represent s.e.m.