Fig. 5.

Znf16l functions cell autonomously in the oligodendrocyte lineage. (A) Diagram of the transgenic constructs driving expression of znf16l by tissue-specific gene promoters in neurons (huC), oligodendrocytes (cldnk, sox10) or macrophages (mpeg). Bottom panel shows another transgenic construct strategy to express mammalian zinc finger proteins, mZfp191 and mZfp488, in oligodendrocytes under the control of the sox10 promoter. (B) Plot quantifies the percentage of mbp rescue using different tissue promoters and different zinc finger proteins. (C) Representative images of mbp rescue in cldnk:znf16l-, sox10:znf16l-, huc:znf16l- and sox10:mZfp488-injected znf16l mutants. There is no visible difference in the level of rescues with the different constructs. Genotypes of all fish analyzed were determined by PCR assay.