Fig. 6.

znf16l and notch3 mutants lack distinct populations of OPCs. In situ hybridization of znf16l^st78, notch3^st51 and notch3^ZM embryos at 54 hpf for deltaD (A-C), notch3 (D-F), and olig2 (G-I) mRNA expression. (A-F) znf16l homozygous mutants do not exhibit decreased deltaD (A) or notch3 (D) expression seen in notch3 homozygous mutants (asterisks in B,C,E). (G′-I′) znf16l mutants exhibit a delay in lateral migration of OPCs (red brackets in G′) that is not seen in either notch3 mutant (H′,I′). (G-I,G″-I″) Lateral view of the hindbrain shows that znf16l mutants are mostly lacking the ventral OPC population (white arrowheads), whereas notch3 mutants are mostly lacking the dorsal OPC population (black arrowheads). Genotypes of all fish analyzed were determined by PCR assay. Scale bars: 100 μm.