Figure 1.

The two-well hanging droplet device, operation, and capabilities. (A) Two 8-device arrays of the two-well hanging droplet device with a close-up image and rendered-model of a single device. (B) Operation of a filled device containing cells, when fluid is removed or added through the interface well, volume of the culture well is changed with minimal disturbance to the culture. (C) Schematic of a dynamic coculutre experiment performed by combining two tissues in the hanging droplet device then separating the tissues.