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. Author manuscript; available in PMC: 2017 Jan 21.
Published in final edited form as: Lab Chip. 2016 Jan 21;16(2):334–344. doi: 10.1039/c5lc01353d

Figure 3.

Figure 3

Effects of pipetting into a single-well and two-well device. (A) Brightfield images of an MM.1S suspension culture in the device before and following 1, 3, and 5 pipetting steps at 1 mL/min of approximately 33% of the total media in single-well and two-well device. (B) PIV was used to determine the velocity of fluid at a fixed z position in the culture droplet during fluid addition to the interface droplet (two-well) or directly (single-well). Error bars represent the standard deviation of four replicates (*p<0.0001).