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. Author manuscript; available in PMC: 2016 Jan 14.
Published in final edited form as: FEBS Lett. 2011 Dec 9;586(1):60–63. doi: 10.1016/j.febslet.2011.11.024

Table 1.

Activities of M. jannaschii SepCysS variants in E. coli

Mj SepCysS Af numbering Relative activity Mj SepCysS Af numbering Activity
WT 100 ± 0 S231A S206 0 ± 0
C64S C39 0 ± 0 H233A H208 0 ± 0
C67S C42 0 ± 0 K234R K209 0 ± 0
R96A R73 89 ± 27 K265A K240 0 ± 0
R102A R79 0 ± 0 C272S C247 0 ± 0
H126A H103 0 ± 0 R292A R267 99 ± 51
Y127A Y104 35 ± 17 H325A H300 124 ± 28
D182A D157 107 ± 22 K354A/R356A K329/R331 92 ± 47
N187A N162 85 ± 17 K370A K345 33 ± 22
N208A N183 101 ± 24

SepCysS activities were determined using a benzyl viologen assay. Relative activities were assigned to SepCysS variants based on the color intensity of three repeats with standard deviations indicated.