Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vitro antigen specific T cell proliferation and cytokine production. 1×104 FBS+ or FBS− BM-DC pulsed with 1040-55 mimepitope peptide were used to stimulate 1×105 BDC2.5 CD4+ splenocytes in both FBS+ and FBS− culture media for 96 hours. A. Tritiated thymidine uptake was measured during the final 18 hours of incubation. B–D. Supernatant was removed after 72 hours of incubation and the indicated cytokines were measured with a multiplex cytokine immunoassay.
