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Splenic recall response of non-diabetic mice 20 weeks post BMDC treatment. A–E. 1×106 spleen cells from FBS+ BM-DC, FBS− BM-DC or PBS treated mice were re-stimulated with GAD217-236 peptide for 4 days. A. Tritiated thymidine uptake was measured for the final 16 hours of culture. B–E. Supernatant was removed after 72 hours of culture and the indicated cytokines were measured with a multiplex cytokine immunoassay.
