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. 2016 Jan 8;9:231–241. doi: 10.2147/OTT.S96710

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© 2016 Ge et al. This work is published and licensed by Dove Medical Press Limited

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dsP53-285 suppresses Cyclin D1–CDK4/6 expression mainly by activating p53.

Notes: LNCaP and DU145 cells were transfected with the indicated dsRNAs at 50 nM for 72 hours. (A) Expression of Cyclin D1–CDK4/6 mRNA in both PCa cell lines was analyzed by real-time PCR. GAPDH served as a loading control. *P<0.05 and **P<0.01 compared with the dsP53-285 group. (B) Western blotting was performed to detect the expression of Cyclin D1 and CDK4/6 protein. α-Tubulin served as a loading control.

Abbreviations: CDK, cyclin-dependent kinase; dsRNA, double-stranded RNA; mRNA, messenger RNA; PCa, prostate cancer; PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.