Table 1.

Oligonucleotide sequences for barcoded ChIP-Seq, with 4-plex [13] and 12-plex [44] sequencing designs

Barcode	Forward/reverse	Sequence (5′ → 3′)
ACGT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTACGT CGTAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG
CATT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTCATT ATGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG
GTAT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTAT TACAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG
TGCT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTGCT GCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG
ATCACGT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTATCACGT CGTGATAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
CGATGTT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGATGTT ACATCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
TTAGGCT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTTAGGCT GCCTAAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
TGACCAT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTGACCAT TGGTCAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
ACAGTGT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTACAGTGT CACTGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
GCCAATT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTGCCAATT ATTGGCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
CAGATCT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTCAGATCT GATCTGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
ACTTGAT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTACTTGAT TCAAGTAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
GATCAGT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTGATCAGT CTGATCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
TAGCTTT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTAGCTTT AAGCTAAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
GGCTACT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGCTACT GTAGCCAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG
CTTGTAT	Forward^a Reverse^b	ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTTGTAT TACAAGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG

Phosphorothioate bond between second-to-last and last (final “T” overhang) nucleotides

5′ ends are phosphorylated