Table 1.

Comparison of tumors’ localization by various imaging methods and confirmed with necropsy in mice injected with MPC cells

Tumors’ localization
	Adrenal	Ovary	Lung	Liver	Subcutaneous
MicroCT	0	0	0	0	NA
MRI	9 in 5 mice	4 in 4 mice	>20 in 2 mice	> 50 in 9 mice	NA
[18F]-DA	8 in 4 mice	4 in 4 mice	0	30-40 in 8 mice	0
[18F]-DOPA	9 in 5 mice	4 in 4 mice	1 in 1 mouse	>50 in 9 mice	4 in 4 mice
Necropsy	18 in 9 mice	4 in 4 mice	>20 in 2 mice	>50 in 9 mice	4 in 4 mice
Mouse name	Necropsy and histopathology confirmation
1	Multiple liver tumors (10, approx. 2–4 mm diameter); 2 enlarged adrenal glands, 2.5 mm in diameter; 1 ovarian tumor, 3 mm in diameter.
2	Multiple liver tumors (>10, ranging from 1–5 mm diameter), 2 enlarged adrenal glands, 2.5 mm in diameter; 1 ovarian tumor, 4 mm in diameter.
3	Single liver lesion (2.5 mm in diameter), enlarged adrenal glands, 2 mm in diameter.
4	Multiple liver tumors (>10, ranging from 1–4 mm diameter), enlarged adrenal glands, 2 mm in diameter.
5	Multiple liver tumors (>20, ranging from 1–3 mm diameter), 1 adrenal gland tumor, 3 mm in diameter.
6	Multiple liver tumors (>10, ranging from 1–5 mm diameter), 2 adrenal gland tumors, 3 and 5 mm in diameter; 1 bone tumor, 5 mm in diameter; 1 lung tumor, 2 mm in diameter.
7	Multiple liver tumors (10, ranging from 1–3 mm diameter), 1 ovarian tumor, 4 mm in diameter; 2 adrenal gland tumors, 3 mm in diameter.
8	Multiple liver tumors (>5, ranging from 1–5 mm diameter), 2 adrenal gland tumors, 3 mm in diameter.
9	Multiple liver tumors (>20, ranging from 1–3 mm diameter); 1 ovarian tumor; 3 mm in diameter; 2 adrenal gland tumors; 3 mm in diameter; approx. 20 lung tumors, 0.5 mm in diameter.
All enlarged adrenal glands were confirmed as pheochromocytomas.

MicroCT, MRI and PET imaging were performed at Week 5. All imaging modalities followed by necropsy were performed within a 24 h time window in all nine mice.