Table 3. Effect of media in the sample preparation procedure on X. fragariae detection after 21 h incubationa.
| Bacteria-spiked | Inoculated tissue | |||||||
|---|---|---|---|---|---|---|---|---|
| qPCR | LAMP | qPCR | LAMP | |||||
| Media | LS-means | LS-means | LS-means | LS-means | ||||
| H2O | 36.8 | A | 40.5 | A | 39.5 | A | 45.0 | A |
| SPB | 36.8 | A | 36.0 | A | 34.5 | B | 42.3 | B |
| R2A | 32.7 | B | 20.4 | B | 35.0 | B | 45.0 | A |
| PBS | 30.5 | C | 12.5 | C | 33.7 | C | 21.9 | C |
a Shown are the LS-means of the Ct (qPCR) and Tt (LAMP) values for each media at 21 h after incubation of 104 CFU/mL of X. fragariae with healthy strawberry leaf or petiole tissues (bacteria-spiked) and 21 h after incubation of spray-inoculated strawberry leaf samples.
The standard errors are 1.02 (qPCR) and 2.81 (LAMP) for the bacteria-spiked samples, and 0.225 (qPCR) and 0.725 (LAMP) for the inoculated samples.