Table 2.
Benchmarking primers used to assess performance and versatility of newly designed primers.
| Locus | Primer | Oligo nucleotides (5’-3’) | Reference |
|---|---|---|---|
| ITS | ITS5 | GGAAGTAAAAGTCGTAACAAGG | Ward & Adams (1998) |
| ITS4 | TCCTCCGCTTATTGATATGC | White et al. (1990) | |
| ITS1 | TCC GTA GGT GAA CCT GCG G | White et al. (1990) | |
| ITS1-F | CTT GGT CAT TTA GAG GAA GTA A | Gardes & Bruns (1993) | |
| LSU | LROR | ACCCGCTGAACTTAAGC | Vilgalys & Hester (1990) |
| LR5 | TCCTGAGGGAAACTTCG | ||
| TUB2 | Btub2Fd | GTBCACCTYCARACCGGYCARTG | Woudenberg et al. (2009) |
| Btub4Rd | CCRGAYTGRCCRAARACRAAGTTGTC | ||
| Bsens | ATCACWCACTCICTIGGTGGTGG | Lesage-Meessen et al. (2011) | |
| Brev | CATGAAGAARTGIAGACGIGGG | ||
| ACT | ACT512f | ATGTGCAAGGCCGGTTTCG | Carbone & Kohn (1999) |
| ACT783r | TACGAGTCCTTCTGGCCCAT | ||
| CA5R | GTGAACAATGGATGGACCAGATTCGTCG | Daniel et al. (2001), Daniel & Meyer (2003) | |
| CA14 | AACTGGGATGACATGGAGAAGATCTGGC | ||
| RPB2 | fRPB2-5f | GAY GAY MGW GAT CAY TTY GG | Liu et al. (1999), Binder & Hibbett (Clark University website) |
| fRPB2-7cF | ATG GGY AAR CAA GCY ATG GG | ||
| fRPB2-7cR | CCC ATR GCT TGY TTR CCC AT | ||
| TEF1α | EF1-983F | GCY CCY GGH CAY CGT GAY TTY AT | Rehner & Buckley (2005) |
| EF1-1567R | ACH GTR CCR ATA CCA CCR ATC TT |