Table 3.
Initial seed primers from ‘nucleic acid based’ computational predictions (Robert et al. 2011).
| Alignment group | Species in alignment (Al) | Primers (forward-reverse) | Functional annotation |
|---|---|---|---|
| 1 | 51 | acaagcgtttct-catcaagttcca | Hypothetical protein |
| 2 | 41 | acatggagaaga-catcaaggagaa | Gamma actin |
| 3 | 44 | accttcttgatg-atgttcttgatg | Translation elongation factor 1α |
| 3b | 46 | caagaacatgat-catcaagaaggt | Translation elongation factor 1α |
| 4 | 38 | agtacttgtagg-cttggccttgta | 60S ribosomal protein L15b |
| 5 | 51 | ggaacttgatgg-agaaacgcttgt | 60S ribosomal protein L15b |
| 6 | 45 | ggtatcaccatc-caacaagatgga | Translation elongation factor 1α |
| 7 | 45 | tccatcttgttg-gatggtgatacc | Translation elongation factor 1α |
| 8 | 36 | gtccatcttgtt-tacttgaaggaa | Translation elongation factor 1α |
| 9 | 56 | gttcttggagtc-gtccatcttgtt | Translation elongation factor 1α |
| 10 | 56 | aacaagatggac-ctccaagaacga | Translation elongation factor 1α |
| 11 | 41 | aacaagatggac-tcaccactgaag | Translation elongation factor 1α |
| 11b | 42 | tggtatctccaa-aacgtcaagaac | Translation elongation factor 1α |
| 12 | 58 | caacaagatgga-ctccaagaacga | Translation elongation factor 1α |
| 13 | 42 | cacttcttcatg-atggacgagatg | 60S ribosomal protein L15b |
| 14 | 37 | catatgcttgtc-gactcgtcatct | Putative CD box sno-RNA protein,putative protein S6 kinase |
| 15 | 43 | cttcagtggtga-ccatcttgttga | Translation elongation factor 1α |
| 16 | 51 | gaacttgatggt-agaaacgcttgt | Hypothetical protein |
| 17 | 36 | gttccttcaagt-caacaagatgga | Hypothetical protein |
| 18 | 36 | tccatcttgttg-acttgaaggaac | Translation elongation factor 1α |
| 19 | 48 | tcttgacgttga-gtccatcttgtt | Translation elongation factor 1α |
| 20 | 48 | ttcttgacgttg-gtccatcttgtt | Translation elongation factor 1α |
| 21 | 39 | tcgttcttggag-tcttgatgaagt | Translation elongation factor 1α |
| 21b | 53 | ttcttggagtca-tccatcttgttg | Translation elongation factor 1α |
| 22 | 45 | ttcttgacgttg-catgttcttgat | Translation elongation factor 1α |
| 23 | 37 | ttccttcaagta-caacaagatgga | Translation elongation factor 1α |
| 24 | 45 | ttcatcaagaac-tcaacgtcaaga | Translation elongation factor 1α |
| 25 | 41 | ttcagtggtgac-atcatgttcttg | Translation elongation factor 1α |
| 26 | 39 | tccttgatttcg-cttcatgacctt | Translation elongation factor 1α |
| 27 | 37 | tcaagaaggtcg-ctccaagaacga | Translation elongation factor 1α |
| 28 | 46 | tcaagaacatga-caacgtcaagaa | Translation elongation factor 1α |
| 29 | 40 | caacaagatgga-aagttcatcaag | Translation elongation factor 1α |
| 30 | 47 | gttcttgacgtt-gtccatcttgtt | Translation elongation factor 1α |
| 31 | 48 | aacaagatggac-caacgtcaagaa | Translation elongation factor 1α |
| 32 | 39 | gacttgatgaac-ccatcttgttga | Translation elongation factor 1α |
| 33 | 45 | catcaagaacat-tcaacgtcaaga | Translation elongation factor 1α |
| 34 | 52 | catcaagaacat-gactccaagaac | Translation elongation factor 1α |
| 35 | 40 | catcaagaaggt-gactccaagaac | Translation elongation factor 1α |
| 36 | 40 | atcaagaacatg-tcaccactgaag | Translation elongation factor 1α |
| 37 | 51 | caagcgtttctc-ccatcaagttcc | Translation elongation factor 1α |
| 38 | 39 | atctccaaggat-ctccaagaacga | Translation elongation factor 1α |
| 39 | 52 | atcaagaacatg-gactccaagaac | Translation elongation factor 1α |
| 40 | 45 | atcaagaacatg-tcaacgtcaaga | Translation elongation factor 1α |
| 41 | 40 | acttgatgaact-tccatcttgttg | Translation elongation factor 1α |
| 42 | 37 | acttcatcaaga-tcaacgtcaaga | Translation elongation factor 1α |
| 43 | 40 | acaagatggaca-gactccaagaac | Translation elongation factor 1α |
| 44 | 38 | aacaagatggac-aagttcatcaag | Translation elongation factor 1α |
| 45 | 39 | aaggtcatgaag-cgaaatcaagga | Translation elongation factor 2 |
| 46 | 42 | catctcgtccat-catgaagaagtg | Beta tubulin 2 |
| 47 | 45 | gttcttgaactt-cttcatcttcca | Translation elongation factor 3 |
| 48 | 39 | tccttgatttcg-ccatcttggaga | Translation elongation factor 3 |
| 49 | 45 | tggaagatgaag-aagttcaagaac | Translation elongation factor 3 |
| 50 | 42 | tggaagatgaag-tgtcaagaccaa | Translation elongation factor 3 |
| 51 | 42 | ttggtcttgaca-cttcatcttcca | Translation elongation factor 3 |
| 52 | 51 | tggaacttgatg-gagaaacgcttg | 60S ribosomal protein L10 (L1) |
| 53 | 35 | ggtatcaccatc-ctccaagaacga | Translation elongation factor 1α |
| 54 | 40 | ttcatcaagaac-tcaccactgaag | Translation elongation factor 1α |
Seed primer sequences highlighted bold, were those qualifying for the last and final cross-laboratory trial.