Table 6.
Super primers and best candidate primers inferred from ‘nucleic acid based’ computational predictions (Robert et al. 2011).
| Locus | Original primer name | Final primer name | Sequence (5’-3’) |
|---|---|---|---|
| TEF1α | Al33_54_73_F1_forward | EF1-1018F | GAYTTCATCAAGAACATGAT |
| Al33_879_859_R2_reverse | EF1-1620R | GACGTTGAADCCRACRTTGTC | |
| Al_34_EF1_300_F1_forward | EF1-1002F | TTCATCAAGAACATGAT | |
| AL34_EF1_1050_R_Tail_reverse | EF1-1688R | GCTATCATCACAATGGACGTTCTTGGAG | |
| Al33_129_148_F2_forward | Al33_alternative_f | GARTTYGARGCYGGTATCTC | |
| Al28_EF1_400_f | EF1_alterantive_3f | TTYGARGCYGGTATCTC | |
| Al28_EF1_900_R | EF1_alternative_3r | GAVACRTTCTTGACGTTGAA | |
| 60S L10 (L1) | AlGr52_412-433_f1 | 60S-908R | CTTVAVYTGGAACTTGATGGT |
| Algr52_1102_1084_R1 | 60S-506F | GHGACAAGCGTTTCTCNGG | |
| TEF3 | Al50+51_EF3_2900_f | EF3-3185F | TCYGGWGGHTGGAAGATGAAG |
| Al50+51_EF3_3300_R | EF3-3538R | YTTGGTCTTGACACCNTC | |
| Al47_EF3_1650_forward | EF3-3188F | GGHGGHTGGAAGATGAAG | |
| A47_EF3_2451_R1_reverse | EF3-3984R | TCRTAVSWGTTCTTGAACTT | |
| Al49_ EF3_44_63_F1_forward | EF3-3186F | CYGGHGGHTGGAAGATGAAG | |
| Al49_ EF3_846_829_r1_reverse | EF3-3984R2 | TCRTAVSWGTTCTTGAAC |