Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 15;27(2):247–259. doi: 10.1091/mbc.E14-11-1555

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Borinskaya et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 6: — N-WASP overexpression changes shape of Nck SH3–induced actin structures. (A–C) Actin structures induced by aggregation of Nck SH3 (A, C) or VCA (B) in NIH3T3 cells (A, B) or NIH3T3 cells overexpressing mVen-N-WASP (C). Cells were fixed. (C) mVen-N-WASP (top left), merge of mTFP1-actin (green) and Nck SH3 clusters (red; top right), CD16/7-mCherry-Nck (bottom left), and mTFP1-actin (bottom right). Scale bars, 10 μm. (D) Morphology analysis of Nck SH3–induced actin structures in the control and in cells overexpressing N-WASP. Nck-SH3: three cells, 200 aggregates; Nck-SH3 plus N-WASP overexpression: three cells, 329 aggregates; VCA: three cells, 250 aggregates. Error bars are mean ± SEM. *p < 0.001.