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. 2016 Jan 15;27(2):260–276. doi: 10.1091/mbc.E15-02-0070

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© 2016 Copeland et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 11: — FHDC1-induced Golgi dispersion is unaffected in centrinone-treated cells. NIH 3T3 cells were treated with the Plk4 inhibitor centrinone (300 nM) for 14 d, and the effects of this treatment on FHDC1-induced Golgi dispersion were assessed by immunofluorescence. (A) mCherryFP-tagged FHDC1 (red) was expressed by transient transfection in control cells that were subsequently stained with anti-giantin (white) and anti–γ-tubulin (green) antibodies. (B) mCherryFP-tagged FHDC1 (red) was expressed by transient transfection in centrinone-treated cells that were subsequently stained with anti-giantin (white) and anti–γ-tubulin (green) antibodies. FHDC1 expression is sufficient to induce Golgi dispersion in both cases, even though the centrosome can no longer be detected in centrinone-treated cells. Scale bar, 10 μm. (C) Quantification of data in A and B. Centrinone treatment did not modify the effects of FHDC1 and FHDC1.I180A expression on Golgi dispersion. N = 3, >100 cells counted per experiment; error bars indicate SEM.