FIGURE 12:

FHDC1-induced Golgi dispersion is inhibited in GM130-depleted cells. NIH 3T3 cells were transfected with (A) control siRNA duplexes or (B) duplexes targeting GM130 to knock down GM130 expression. Distribution of endogenous FHDC1 protein (red) is not affected in control cells but is less filamentous and more punctate in GM130-knockdown cells (green). Full-length FHDC1 (red) was transiently expressed in (C) control and (D) GM130-knockdown cells. FHDC1-induced Golgi dispersion is unaffected in control cells but is inhibited in GM130-depleted cells. Scale bar, 10 μm. (E) Quantification of the data in C and D. GM130 knockdown did not modify the effects of FHDC1.958N expression on Golgi morphology. N = 3, >100 cells counted per experiment. Error bars, SEM. (F) Whole-cell lysates from GM130-knockdown, control transfected cells and untransfected cells were immunoblotted for GM130, FHDC1, and α-tubulin. Endogenous FHDC1 expression is not affected by GM130 depletion.