FIGURE 4:

Brefeldin A treatment decreases FHDC1 microtubule localization. (A) In untreated cells, FHDC1 is found primarily on the microtubule network and in close association with the Golgi. (B) After 45 min of treatment with 5.0 μg/ml brefeldin A, FHDC1 subcellular localization in many cells is less filamentous and has a more punctuate and diffuse cytoplasmic distribution, as typified by the indicated cell (arrow). (C) At15 min after removal of brefeldin A, the Golgi ribbon has begun to reassemble; FHDC1 is regaining a more filamentous distribution and is concentrated at the Golgi. (D) In fully recovered cells, FHDC1 is primarily filamentous and preferentially associated with the Golgi. Scale bar, 10 μm. (E) Quantification of data shown in A–D. In untreated cells, FHDC1 is primarily filamentous (blue bars). In contrast, after 45 min of treatment with brefeldin A, FHDC1 exhibits a more punctuate distribution. N = 3, >100 cells counted per experiment. Error bars indicate SEM.