Extended Data Figure 6. Evidence for retention of important catalytic motifs and recurrent eukaroytic-specific addition of secretion signals.

a-c, Alignments for the predicted Dae N-terminal signal sequences (shaded blue) and catalytic motifs (right) are shown for each of the families. The consensus sequence logo of residues surrounding the cysteine and histidine positions of catalytic dyads from extant Tae enzymes are shown above alignments from each family. Below are aligned eukaroytic Dae proteins in these same regions. Representatives derived from distinct HGT events are separated by a space. Predicted N-terminal secretion signals (blue) and predicted catalytic residues (red) are colored. Lowering the cutoff value in SignalP³⁶ from the default value of 0.45 to the 'sensitive' value of 0.34 predicted a signal peptide in residues 1-21 of C. gigas Dae4.