Table 1. Genetic analysis of the roles of cdc-42 and gdi-1 during AC invasion.
| Genotype/Treatment | Full invasion± | Partial invasion | No invasion | n = |
|---|---|---|---|---|
| Secondary RNAi screen [human orthologs listed in brackets] | ||||
| unc-40(e271) [Deleted in colon cancer (DCC)] | 28% | 17% | 54% | 150 |
| mig-6 [Papilin] RNAi; unc-40(e271)** | 4% | 7% | 89% | 150 |
| gdi-1 [Rab GDIβ] RNAi; unc-40(e271)** | 5% | 15% | 80% | 150 |
| gei-4 RNAi [LOC283658 portein]; unc-40(e271)* | 6% | 8% | 86% | 50 |
| cdc-42 RNAi [CDC42]; unc-40(e271)** | 7% | 11% | 83% | 150 |
| gpr-1 [n/a] RNAi; unc-40(e271)** | 9% | 13% | 77% | 150 |
| F09A5.4 [n/a] RNAi; unc-40(e271)** | 9% | 19% | 71% | 150 |
| mel-11 [n/a] RNAi; unc-40(e271)** | 9% | 24% | 67% | 150 |
| gpr-2 [n/a] RNAi; unc-40(e271)** | 10% | 18% | 72% | 150 |
| gtr-1 [n/a] RNAi; unc-40(e271)** | 10% | 19% | 71% | 150 |
| gsa-1 [GNAS] RNAi; unc-40(e271)** | 11% | 12% | 77% | 150 |
| pat-4 [Integrin-linked kinase] RNAi; unc-40(e271)* | 12% | 17% | 71% | 150 |
| Y70D2A.1 [GPR139] RNAi; unc-40(e271)* | 12% | 20% | 68% | 150 |
| lin-3 [n/a] RNAi; unc-40(e271) | no vulval precursor cells | |||
| Tissue-specific RNAi experiments | ||||
| Uterine-specific RNAi control | 92% | 6% | 2% | 100 |
| Uterine-specific wsp-1 RNAia | 41% | 27% | 32% | 100 |
| Uterine-specific gdi-1 RNAia | 44% | 23% | 33% | 100 |
| Vulval-specific RNAi control | 98% | 2% | 0% | 50 |
| Vulval-specific gdi-1 RNAib | 96% | 4% | 0% | 45 |
| Rescue and Genetic interaction experiments | ||||
| Wild type | 100% | 0% | 0% | >100 |
| cdc-42(gk388)c | 66% | 10% | 24% | 50 |
| cdc-42 RNAic,d | 72% | 15% | 14% | 74 |
| cdc-42(gk388); cdc-42 RNAic,d | 68% | 8% | 24% | 25 |
| cdc-42(gk388); qyIs410[cdh-3>GFP:cdc-42]e | 92% | 4% | 4% | 25 |
| wsp-1(gm324)c | 18% | 28% | 54% | 50 |
| gdi-1 RNAic | 57% | 13% | 30% | 30 |
| qyEx533 [cdh3>GFP::Cbrgdi-1]; gdi-1 RNAif | 91% | 5% | 4% | 57 |
| gdi-1 RNAi; cdc-42 RNAiff | 35% | 35% | 29% | 31 |
| cdc-42(gk388); gdi-1 RNAidd | 5% | 18% | 78% | 40 |
| lin-3 RNAi | 18% | 13% | 69% | 100 |
| lin-3 RNAi; cdc-42(gk388)g | 18% | 12% | 70% | 100 |
| lin-3(n378)/lin-3(1059) | 21% | 10% | 69% | 100 |
| gdi-1 RNAi; lin-3(n378)/lin-3(1059)h | 20% | 13% | 67% | 100 |
±Full invasion, partial invasion, and no invasion were defined by the degree to which the BM beneath the AC was cleared, as previously described [21].
Statistical comparisons of scoring (Fisher's exact test, normal invasion versus defective invasion):
*Compared with unc-40(e271); p<0.001
**Compared with unc-40(e271); p<0.0001
aCompared with uterine-specific RNAi control; p<0.0001
bCompared with vulval-specific RNAi control; not significant
cCompared with wild type; p<0.0001
dCompared with cdc-42(gk388); not significant
eCompared with wild type; not significant; heterozygous qyIs410 rescue
ddCompared with cdc-42(gk388); p<0.0001
fCompared with gdi-1 RNAi; p<0.001
ffCompared with gdi-1 RNAi; not significant
gCompared with lin-3 RNAi; not significant
hCompared with lin-3(n378)/lin-3(1059); not significant